Cell Therapy / Oncology

Lifileucel (lifileucel)

Iovance Biotherapeutics · Tumor-Infiltrating Lymphocyte (TIL) Therapy

FDA Approved 2024 IV infusion (autologous, patient-specific) Specialty Medication
Lifileucel Amtagvi tumor-infiltrating lymphocyte TIL therapy autologous cryo-preserved infusion bag, Iovance Biotherapeutics

What is Lifileucel? Lifileucel (brand: Amtagvi) is the first FDA-approved tumor-infiltrating lymphocyte (TIL) therapy, approved for adult patients with unresectable or metastatic melanoma who have received prior treatment with a PD-1/PD-L1 blocking antibody and a BRAF inhibitor (if BRAF V600 mutation positive).

About Lifileucel

Brand NameLifileucel
Generic / INNlifileucel
Drug ClassTumor-Infiltrating Lymphocyte (TIL) Therapy
ManufacturerIovance Biotherapeutics
AdministrationIV infusion (autologous, patient-specific)
FDA Approval2024
📦 Patient-specific autologous product — no standard NDC. Each batch is manufactured from the individual patient's tumor tissue.

FDA-Approved Uses for Lifileucel

Indications are based on FDA approval. Your healthcare provider determines appropriate use based on your specific diagnosis, stage, biomarker status, and overall clinical picture.

  • Unresectable or metastatic melanoma — adult patients after prior treatment with a PD-1/PD-L1 checkpoint inhibitor and a BRAF inhibitor (if BRAF V600 mutation-positive)
Biomarker Note: BRAF V600 mutation status must be tested before starting lifileucel to determine if prior BRAF inhibitor therapy was required. Patients must have received prior anti-PD-1/PD-L1 therapy.

How Does Lifileucel Work?

Lifileucel consists of tumor-infiltrating lymphocytes (TILs) — immune cells naturally present within the patient's own tumor. TILs are harvested from a resected tumor fragment, expanded ex vivo to large numbers (up to billions of cells), and infused back into the patient after lymphodepleting chemotherapy. The expanded TILs have diverse natural T-cell receptors recognizing tumor-specific antigens and attack melanoma cells without genetic engineering.

Dosage & Administration

Single IV infusion following non-myeloablative lymphodepleting chemotherapy (fludarabine + cyclophosphamide), then high-dose interleukin-2 (aldesleukin) after infusion. The number of cells infused is patient-specific (minimum 7.5 × 10⁹ viable cells). The full process takes approximately 5–7 weeks from tumor harvest to infusion.

⚠️ Important

Dosing is individualized by the prescribing clinician. Never adjust your dose without consulting your healthcare provider. This information is educational only and does not replace the official prescribing information.

Warnings, Precautions & Side Effects

General Warning

Cytokine release syndrome (CRS). Prolonged severe cytopenias. Severe infections. Prolonged immune deficiency. Respiratory failure. High-dose IL-2 toxicity (required post-infusion): capillary leak syndrome, hypotension, pulmonary edema. Neurological toxicity. Available only at certified healthcare facilities.

⛔ Serious Warnings & Precautions
  • Cytokine release syndrome
  • Prolonged severe cytopenias
  • Severe infections
  • Respiratory failure
  • High-dose IL-2 capillary leak syndrome
  • Neurological toxicity

Common Side Effects

  • CRS
  • Prolonged cytopenias
  • Infections
  • Nausea
  • Fatigue
  • IL-2 toxicity effects
  • Hypotension

This is not a complete list. Always report new or worsening symptoms to your healthcare provider promptly.

Storage & Handling

Cryopreserved product — stored in liquid nitrogen vapor phase (≤ −130°C). Must be shipped and handled under validated cryogenic conditions.

BiologicHealthPlus provides temperature-monitored cold-chain specialty delivery for medications requiring refrigeration. Never use a medication if its storage conditions have been compromised.

Insurance Coverage, Prior Authorization & Patient Assistance

🛡️ Commercial Insurance

Lifileucel is covered by most major commercial insurance plans when medically necessary and prior authorized. Coverage tier and cost-sharing vary by plan.

✅ Prior Authorization

Prior authorization is required by virtually all insurance plans for Lifileucel. BiologicHealthPlus handles the complete prior authorization process at no cost to patients or providers.

🏥 Medicare Coverage

Medicare Part B covers this IV medication when administered in a clinical setting. Medicaid coverage varies by state.

🆓 Patient Assistance

Manufacturer patient assistance programs may be available for eligible uninsured or underinsured patients. Contact BiologicHealthPlus for a confidential eligibility review.

Prior Authorization Patient Assistance Insurance & Savings Medication Costs

Frequently Asked Questions — Lifileucel

Lifileucel (Amtagvi) is the first FDA-approved tumor-infiltrating lymphocyte (TIL) therapy, approved in 2024 for unresectable or metastatic melanoma after prior checkpoint inhibitor therapy (and BRAF inhibitor if BRAF-mutated).

CAR-T cells are genetically engineered to express a specific synthetic receptor targeting one antigen. TIL therapy uses the patient's own naturally occurring tumor-reactive T cells — harvested from the tumor itself — which have diverse natural T-cell receptors and can recognize multiple tumor antigens simultaneously.

High-dose interleukin-2 (IL-2/aldesleukin) is given after the TIL infusion to expand and sustain the infused lymphocytes. IL-2 causes significant toxicities (capillary leak, hypotension) that require intensive monitoring at certified facilities.

As a 2024 FDA approval, coverage is still being established across plans. Prior authorization with full documentation of prior therapy (checkpoint inhibitor + BRAF inhibitor if applicable) is required. BiologicHealthPlus assists with access coordination.

Related Medications

Keytruda (pembrolizumab)  ·  Opdivo (nivolumab)  ·  Tafinlar + Mekinist (BRAF/MEK)  ·  Yescarta  ·  Abecma

Related Conditions

Unresectable melanoma  ·  Metastatic melanoma  ·  Advanced skin cancer

Comparisons

Abecma Yescarta

Authoritative References

Medical Disclaimer: This page is for general educational purposes only and does not constitute medical advice, diagnosis, or treatment. Always consult your oncologist, hematologist, or licensed healthcare professional before making any treatment decisions. Last reviewed: August 2026 by the BiologicHealthPlus Clinical Team.